Device

Part:BBa_K199157

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-05-11)


pLac-RBS-CCACU-RFP-pLpp-CUAGU tRNA-pLpp-CCACU tRNA

This is a device that tests the production of the 2nd tRNA in a construct when each tRNA has its own promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1003
    Illegal BamHI site found at 1211
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 786
    Illegal AgeI site found at 898
    Illegal AgeI site found at 1040
    Illegal AgeI site found at 1248
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLac-RBS-CCACU-RFP-pLpp-CUAGU tRNA-pLpp-CCACU tRNA